機器(委託対応)の詳細情報

Detailed Information of Equipment(Job Request)

Equipment and Job Request Information
Affiliation Chemical Analysis Div., Research Facility Center for Science and Technology
Category Biocompound Analyzers
Equipment (Job Request) Amino Acid Analysis
Supplier (Equipment type) JEOL (JLC-500/V2 Automatic Amino Acid Analyzer)

Specification・Features

[Overview]
Simultaneous analysis of 38 or more amino acids by liquid chromatography using post-column ninhydrin derivatization method. Amino acids in a solution containing peptides and proteins hydrolyzed to amino acids or in a solution containing biological components with proteins removed are analyzed. Compared with the standard sample flowed separately, the type of amino acid and its concentration are quantified (external standard method). Appropriate concentrations for analysis are roughly 100 μM (nmol/mL) of each amino acid. Please adjust the sample to pH 2 to 3 in advance. The sample must be filtered through a 0.22-0.45 μm sterilization filter. Precipitation, turbidity-induced samples are not analyzed. The amount of each sample should be more than 300 μL in 1.5 or 2.0 mL microtube. If you would like to analyze Asparagine, Glutamine, Tryptophan and Norleucine, please consult us and prepare fresh standard samples prepared at 100 μM (nmol/mL), pH 2-3, more than 300 μL.

[About protein hydrolysis]
In this Chemical Analysis Division, protein hydrolysis treatment is not carried out. Depending on the type of amino acid, quantitative analysis may not be possible due to chemical change or racemization in the course of hydrolysis treatment. Please refer to the prior literature, please carefully consider the conditions, then perform hydrolysis treatment.
Installation site Research Facility Center for Science and Technology, Chemical Analysis Division 203
Remark
Research Accomplishment
Inquiry 利用相談はこちらより必要事項をご記入の上、お問い合わせください。
機器担当者 担当者:廣瀬(分析部門203室)
University-wide
Job Request Rate
5,570 Yen / specimen
Picture