機器(委託対応)の詳細情報

Detailed Information of Equipment(Job Request)

Equipment and Job Request Information
Affiliation Laboratory of Sports Medicine, Institute of Medicine
Category Biocompound Analyzers
Equipment (Job Request) Genotyping (mouse, rat, other animal species)
Supplier (Equipment type)  

Specification・Features

Genotyping of mice and rats is performed using the LabDroid Maholo, a humanoid dual-arm robot, for DNA extraction and purification. Alkaline lysis and bead purification methods are used to remove impurities as much as possible, and DNA of a grade suitable for PCR genotyping experiments is purified and genotyped using a real-time PCR system.
Prior discussion on genetic information and primer design is required.

How to submit and return samples:
Please put 1-2 mm of mouse tail tissue in a 96-well plate prepared by us. Then, dispense 160 µl of 50 mM NaOH/well, cover with a seal, and submit. The required 96-well plate and seal can be provided here. Residual DNA can be returned upon request.

Other:
Price is for one primer pair. 300 yen will be added for each additional primer pair (additional PCR reaction).
We may be able to provide services for animal species other than mice and rats, so please contact us.
Installation site Room No. 060, Institute of Medical Sciences
Remark
Research Accomplishment
Inquiry 利用相談はこちらより必要事項をご記入の上、お問い合わせください。
機器担当者 担当者:菅澤
所属:医学医療系
University-wide
Job Request Rate
2,350 Yen / specimen
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