機器(委託対応)の詳細情報

Detailed Information of Equipment(Job Request)

Equipment and Job Request Information
Affiliation Chemical Analysis Div., Research Facility Center for Science and Technology
Category Electromagnetic Analyzers
Equipment (Job Request) MALDI Mass Spectrometer TOF/TOF (TM) 5800system
Supplier (Equipment type) AB SCIEX (TOF/TOF (TM) 5800system)

Specification・Features

MALDI-TOF MS is a matrix-assisted laser desporption ionization-time-of-flight mass spectrometer combining matrix-assisted laser desorption ionization (MALDI) and time-of-flight mass spectrometry (TOFMS). This mass spectrometry method is used to measure the mass of difficult-to-ionize components (refractory substances, less soluble substances). Particularly, it allows accurate mass measurements of biopolymers (proteins and saccharides) are ionized under soft conditions. In MALDI, the analyte and the matrix (a laser-absorbing compound) are mixed together. When an ultraviolet laser beam (Nd: YAG laser with a wavelength of 349 nm) is directed at the mixture, it is ionized. The time of flight over a certain distance is measured in a high vacuum of 10-8 torr to calculate the mass of the sample. There are TOF-MS measurement modes: a linear mode where the ions are accelerated linearly and a reflector mode where they are accelerated linearly and then reflected by an electrostatic mirror.
The features of AB SCIEX TOF/TOF 5800 are the following:
・ Positive and negative ion detection: [positive (+) ions & negative (-) ions; detection]
・ 1000-Hz high speed laser: Ultra-fast ionization
・ The direct laser bombardment mechanism allows (1) high-resolution, high-accuracy MS measurements in a reflector mode [Mw; ≒ 400 to ≒ 5,000 Da]; (2) mass measurements of a few tens of thousands to a few hundreds of thousands Da with low resolution and accuracy in a linear mode [Mw; ≒ 3,000 to ≒ 200,000 Da]; and (3)MS/MS measurements in a reflector mode.
Date of installation: April 23, 2010
Installation site Research Facility Center for Science and Technology,Chemical Analysis Division 106
Remark
Research Accomplishment
Inquiry 利用相談はこちらより必要事項をご記入の上、お問い合わせください。
機器担当者 担当者:(主)権田・(副)廣瀬
University-wide
Shared Use Rate
2,200 Yen / 30 min.
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