機器(委託対応)の詳細情報

Detailed Information of Equipment(Job Request)

Equipment and Job Request Information
Affiliation Faculty of Medicine Core Facility
Category Spectrophotometers
Equipment (Job Request) Fluorometer Qubit 2.0
Supplier (Equipment type) Life Technologies (Qubit® 2.0 Fluorometer)

Specification・Features

The Qubit® 2.0 Fluorometer is an easy-to-use, analytical instrument designed to work seamlessly with the Qubit® assays (previously known as Quant-iT™ assays) for DNA, RNA, and protein quantitation. The integrated design of the instrument and assays results in the Qubit® 2.0 Fluorometer being far more sensitive and accurate than UV absorbance; this helps avoid repetition of experiments due to inaccurate nucleic acid or protein measurements. It is ideal for cloning, sequencing, transfection, qPCR, microarrays, protein gels, Western blots, and protein activity assays.

With the Qubit® 2.0 Fluorometer you get:
・ A platform designed to provide highly accurate and specific measurement of DNA, RNA, or protein
・ State-of-the-art optical components
・ Sophisticated data analysis
・ An intuitive user interface
・ Small sample volume requirement (1–20 µl)
・ Small bench footprint

《Obtain More Accurate Results than with UV Absorbance》
The Qubit® 2.0 Fluorometer is far more sensitive and accurate than UV absorbance, which measures anything absorbing at 260 nm—DNA, RNA, protein, free nucleotides, or excess salts. Moreover, UV spectrophotometry often does not have the sensitivity to accurately measure low concentrations of DNA and RNA. According to the MIQE Publication Guidelines, the preferred method for quantifying RNA uses fluorescent RNA-binding dyes. With the Qubit® Fluorometer, your research is enhanced by more accurate measurements because the dyes in the Qubit® assay kits fluoresce only when bound to the selected molecule—DNA, RNA, or protein—in your sample. This allows you to avoid repeating work due to inaccurate measurements.

Installation date: 2011.5.30
Installation site Room No. 671, Institute of Medical Sciences
Remark
Research Accomplishment
Inquiry 利用相談はこちらより必要事項をご記入の上、お問い合わせください。
機器担当者 担当者:秋山
所属:筑波大学医学医療系医学共通機器室
University-wide
Shared Use Rate
1,060 Yen / 30 min.
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